Overview
Type: Synthetic peptide
Form: Lyophilized powder
CHRM3 Blocking Peptide (#BLP-MR006) is the original antigen used for immunization during Anti-CHRM3 Antibody (#AMR-006) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-CHRM3 Antibody (#AMR-006), (1:200).
2. Anti-CHRM3 Antibody, preincubated with CHRM3 Blocking Peptide (#BLP-MR006).- Human colon (1:50) (Harrington, A.M. et al. (2010) Neurogastroenterol. Motil. 22, 999.).
Expression of CHRM3 in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CHRM3 Antibody (#AMR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CHRM3 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with CHRM3 Blocking Peptide (#BLP-MR006), suppressed staining. Cell nuclei are stained with DAPI (blue).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-CHRM3 Antibody (#AMR-006-GP), (1:400).
3-4. Guinea Pig Anti-CHRM3 Antibody, preincubated with CHRM3 Blocking Peptide (#BLP-MR006).
Expression of Cholinergic receptor muscarinic 3 in rat dorsal pons.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-CHRM3 Antibody (#AMR-006-GP), (1:1000), followed by goat anti-guinea pig-biotin and Streptavidin-Cy3. A. CHRM3 immunoreactivity (red) appears in the locus coeruleus and motor mesencephalic nucleus. B. Pre-incubation of the antibody with CHRM3 Blocking Peptide (#BLP-MR006), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)TLAKRFALKTRSQITKRKR, corresponding to amino residues 461-479 of rat M3 (Accession P08483).
Accession (Uniprot) Number P08483
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
