Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)TLAKRFALKTRSQITKRKR, corresponding to amino residues 461-479 of rat M3 (Accession P08483).
Accession (Uniprot) Number P08483
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-CHRM3 Antibody (#AMR-006), (1:200).
2. Anti-CHRM3 Antibody, preincubated with CHRM3 Blocking Peptide (#BLP-MR006).- Human colon (1:50) (Harrington, A.M. et al. (2010) Neurogastroenterol. Motil. 22, 999.).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-CHRM3 Antibody (#AMR-006-GP), (1:400).
3-4. Guinea Pig Anti-CHRM3 Antibody, preincubated with CHRM3 Blocking Peptide (#BLP-MR006).
Last update: 24/03/2024
