Overview
Type: Synthetic peptide
Form: Lyophilized powder
CHRM4 Blocking Peptide (#BLP-MR004) is the original antigen used for immunization during Anti-CHRM4 Antibody (#AMR-004) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 2), mouse brain membrane (lane 3 and 5) and SH-SY5Y cell lysate (lanes 4 and 6):1,3,4. Anti-CHRM4 Antibody (#AMR-004), (1:200).
2,5,6. Anti-CHRM4 Antibody, preincubated with CHRM4 Blocking Peptide (#BLP-MR004).
Expression of Muscarinic acetylcholine receptor M4 in mouse islands of CallejaImmunohistochemical staining of mouse islands of Calleja (ventral striatum) using Anti-CHRM4 Antibody (#AMR-004). A. M4 mAChR staining (red) appears in soma of neuronal profiles (vertical arrows) and a diffuse halo (horizontal arrows) in the vicinity of the islands of Calleja (IC). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Properties
Sequence
- (C) RTLNPASKWSK, corresponding to amino acid residues 330-340 of rat M4 muscarinic receptor (Accession P08485).
Accession (Uniprot) Number P08485
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
