Overview
Type: Synthetic peptide
Form: Lyophilized powder
CHRM5 (extracellular) Blocking Peptide (#BLP-MR005) is the original antigen used for immunization during Anti-CHRM5 (extracellular) Antibody (#AMR-005) generation. The blocking peptide binds and ‘blocks’ Anti-CHRM5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 5) and brain (lanes 2 and 6) membranes, human A-875 melanoma (lanes 3 and 7) and human MALM-3M malignant melanoma (lanes 4 and 8) cell lysates:1-4. Anti-CHRM5 (extracellular) Antibody (#AMR-005), (1:200).
5-8. Anti-CHRM5 (extracellular) Antibody, preincubated with CHRM5 (extracellular) Blocking Peptide (#BLP-MR005).
Properties
Sequence
- (C)HNATTVNGTPVNHQPLER, corresponding to amino acid residues 7-24 of human M5 muscarinic receptor (Accession P08912).
Accession (Uniprot) Number P08912
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
