Overview
Type: Synthetic peptide
Form: Lyophilized powder
CLC-K Blocking Peptide (#BLP-CL004) is the original antigen used for immunization during Anti-CLC-K Antibody (#ACL-004) generation. The blocking peptide binds and ‘blocks’ Anti-CLC-K primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-CLC-K Antibody (#ACL-004), (1:200).
2. Anti-CLC-K Antibody, preincubated with CLC-K Blocking Peptide (#BLP-CL004).- Rat kidney sections.
Properties
Sequence
- (C)KKAISTLTNPPAPK, corresponding to amino acid residues 674-687 of the rat longer form CLC-K2L (Accession P51802).
Accession (Uniprot) Number P51802
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
