Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CLEC7A/Dectin-1 (extracellular) Blocking Peptide (#BLP-LR062) is the original antigen used for immunization during Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062) generation. The blocking peptide binds and ‘blocks’ Anti-CLEC7A/Dectin-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062), (1:200).
3-4. Anti-CLEC7A/Dectin-1 (extracellular) Antibody, preincubated with CLEC7A/Dectin-1 (extracellular) Blocking Peptide (BLP-LR062).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and mouse J774 macrophage cell line lysate (lanes 2 and 4):1-2. Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062), (1:200).
3-4. Anti-CLEC7A/Dectin-1 (extracellular) Antibody, preincubated with CLEC7A/Dectin-1 (extracellular) Blocking Peptide (BLP-LR062).
Expression of CLEC7A/Dectin-1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062), (1:2400), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed CLEC7A immunoreactivity (green) in astrocytes of the hilus (H). B. Pre-incubation of the antibody with CLEC7A/Dectin-1 (extracellular) Blocking Peptide (BLP-LR062), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.
Expression of CLEC7A/Dectin-1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062), (1:2400), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed CLEC7A immunoreactivity (green) in astrocytes of the hilus (H) and the outer molecular layer (OML). B. Pre-incubation of the antibody with CLEC7A/Dectin-1 (extracellular) Blocking Peptide (BLP-LR062), suppressed staining. Cell nuclei are stained with DAPI (blue).
Multiplex staining of CLEC7A and GFAP in rat hippocampal CA1 regionImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062), (1:600), followed by goat anti-rabbit-AlexaFluor-488 and Guinea Pig Anti-GFAP Antibody (#AFP-001-GP), (1:600), followed by goat anti-guinea pig-AlexaFluor-594. A. CLEC7A immunoreactivity (green) appears in astrocyte profiles. B. GFAP immunoreactivity (red) appears in astrocyte profiles. C. Merge of the two images demonstrates extensive co-localization. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.
Properties
Sequence
- Peptide (C)RNKENHKPTESSLDEK, corresponding to amino acid residues 87 - 102 of mouse CLEC7A (Accession Q6QLQ4).
Accession (Uniprot) Number Q6QLQ4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
