Overview
Type: Synthetic peptide
Form: Lyophilized powder
CLIC1 Blocking Peptide (#BLP-CL021) is the original antigen used for immunization during Anti-CLIC1 Antibody (#ACL-021) generation. The blocking peptide binds and ‘blocks’ Anti-CLIC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human colorectal adenocarcinoma HT-29 (lanes 1 and 5), transplantable human carcinoma cell line T-84 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates:1-4. Anti-CLIC1 Antibody (#ACL-021), (1:200).
5-8. Anti-CLIC1 Antibody, preincubated with CLIC1 Blocking Peptide (#BLP-CL021).
Properties
Sequence
- (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299).
Accession (Uniprot) Number O00299
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
