Overview
Type: Synthetic peptide
Form: Lyophilized powder
CLIC4 Blocking Peptide (#BLP-CL024) is the original antigen used for immunization during Anti-CLIC4 Antibody (#ACL-024) generation. The blocking peptide binds and ‘blocks’ Anti-CLIC4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human pancreatic carcinoma PANC-1 (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat kidney (lanes 3 and 6) lysates:1-3. Anti-CLIC4 Antibody (#ACL-024), (1:200).
4-6. Anti-CLIC4 Antibody, preincubated with CLIC4 Blocking Peptide (#BLP-CL024).
Properties
Sequence
- (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4 (Accession Q9Y696).
Accession (Uniprot) Number Q9Y696
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
