Overview
Type: Synthetic peptide
Form: Lyophilized powder
CNGA2 Blocking Peptide (#BLP-PC045) is the original antigen used for immunization during Anti-CNGA2 Antibody (#APC-045) generation. The blocking peptide binds and ‘blocks’ Anti-CNGA2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CNGA2 Antibody (#APC-045), (1:200).
2. Anti-CNGA2 Antibody, preincubated with CNGA2 Blocking Peptide (#BLP-PC045).
Expression of CNGA2 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-CNGA2 Antibody (#APC-045). A. CNGA2 (red) appears in Purkinje cells (vertical arrows) and in astrocytic fibers (horizontal arrows) traversing the cerebellar molecular layer (Mol). B. Staining of astrocytes with mouse anti-glial fibrillary acidic protein (GFAP, green) demonstrates the full distribution of astrocytic fibers (horizontal arrows) in the cerebellum. C. Confocal merge of panels A and B.
Expression of CNGA2 in mouse cerebrumImmunohistochemical staining of mouse cerebrum using Anti-CNGA2 Antibody (#APC-045). A. CNGA2 (red) appears in cells lining up the wall of the lateral ventricle (LV) (horizontal arrows). B. Staining of astrocytes with mouse anti-glial fibrillary acidic protein (GFAP, green) demonstrates penetration of astrocytic fibers (vertical arrows) into the wall of the lateral ventricle. C. Confocal merge of panels A and B.
Properties
Sequence
- (C)KQNHEDDYLSDGINTPEP, corresponding to amino acid residues 643-660 of rat CNGA2. (Accession Q00195).
Accession (Uniprot) Number Q00195
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
