Overview
Type: Synthetic peptide
Form: Lyophilized powder
CNGA4 Blocking Peptide (#BLP-PC074) is the original antigen used for immunization during Anti-CNGA4 Antibody (#APC-074) generation. The blocking peptide binds and ‘blocks’ Anti-CNGA4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-CNGA4 Antibody (#APC-074), (1:200).
2. Anti-CNGA4 Antibody, preincubated with CNGA4 Blocking Peptide (#BLP-PC074).
Expression of CNGA4 in mouse olfactory bulbImmunohistochemical staining of CNGA4 channel in mouse olfactory bulb using Anti-CNGA4 Antibody (#APC-074). A. CNGA4 staining (green) appears in glomeruli (horizontal arrows) and outside the glomeruli (vertical arrows). To define the location of glomeruli (G), the same section was stained with mouse anti-synaptophysin (red) and the nissl stain DAPI (blue). B. Pre-incubation of Anti-CNGA4 Antibody with the peptide antigen blocks the staining in the olfactory bulb.
Properties
Sequence
- (C) SQDGKVKTTESTPPAPTK, corresponding to amino acid residues 2-19 of rat CNGA4 (Accession Q64359).
Accession (Uniprot) Number Q64359
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
