Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF (Accession P26441).
Accession (Uniprot) Number P26441
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis using Anti-CNTF Antibody on:1. Recombinant human CNTF protein (#C-240), 20ng.
2. Recombinant human Cardiotrophin-1 protein (#C-200), 20ng.
3. Recombinant human LIF protein (#L-200), 20ng.
4. mIL-6, 20 ng.
5. Recombinant human CNTF protein (#C-240), 20ng.
Lanes 1-4. Anti-CNTF Antibody (#ANT-031), (1:200).
Lane 5. Anti-CNTF Antibody, preincubated with CNTF Blocking Peptide (#BLP-NT031).
Antibody specificity is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6.- Rat brain sections (frozen), (1:200).
Last update: 02/01/2024
