Overview
Type: Synthetic peptide
Form: Lyophilized powder
Connexin-26/GJB2 Blocking Peptide (#BLP-CC212) is the original antigen used for immunization during Anti-Connexin-26 (GJB2) Antibody (#ACC-212) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-26/GJB2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes:1,2. Anti-Connexin-26 (GJB2) Antibody (#ACC-212), (1:200).
3,4. Anti-Connexin-26 (GJB2) Antibody, preincubated with Connexin-26/GJB2 Blocking Peptide (#BLP-CC212).- Human fetal cochlear sections (Locher, H. et al. (2015) Dev. Neurobiol. 75, 1219.).
Properties
Sequence
- (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994).
Accession (Uniprot) Number P21994
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
