Cat #: BLP-CC212
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994).
Accession (Uniprot) Number P21994
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes:1,2. Anti-Connexin-26 (GJB2) Antibody (#ACC-212), (1:200).
3,4. Anti-Connexin-26 (GJB2) Antibody, preincubated with Connexin-26/GJB2 Blocking Peptide (#BLP-CC212). - Human fetal cochlear sections (Locher, H. et al. (2015) Dev. Neurobiol. 75, 1219.).
Last update: 02/01/2024