Overview
Type: Synthetic peptide
Form: Lyophilized powder
Connexin-36 Blocking Peptide (#BLP-CC209) is the original antigen used for immunization during Anti-Connexin-36 Antibody (#ACC-209) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-36 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-Connexin-36 Antibody (#ACC-209), (1:200).
3,4. Anti-Connexin-36 Antibody, preincubated with Connexin-36 Blocking Peptide (#BLP-CC209).
Expression of Connexin-36 in rat spinal cordImmunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-Connexin-36 Antibody (#ACC-209), (1:300). Cx36 staining (in red) appears in processes (arrows) along the first layer of the dorsal horn of the rat spinal cord. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36 (Accession O70610).
Accession (Uniprot) Number O70610
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
