Overview
Type: Synthetic peptide
Form: Lyophilized powder
Connexin-37 Blocking Peptide (#BLP-CC204) is the original antigen used for immunization during Anti-Connexin-37 Antibody (#ACC-204) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-37 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse heart lysate (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and mouse lung lysate (lanes 3 and 6):1-3. Anti-Connexin-37 Antibody (#ACC-204), (1:400).
4-6. Anti-Connexin-37 Antibody, preincubated with Connexin-37 Blocking Peptide (#BLP-CC204).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate:1. Anti-Connexin-37 Antibody (#ACC-204), (1:200).
2. Anti-Connexin-37 Antibody, preincubated with Connexin-37 Blocking Peptide (#BLP-CC204).
Properties
Sequence
- (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37 (Accession Q03190).
Accession (Uniprot) Number Q03190
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
