Cat #: BLP-CC204
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37 (Accession Q03190).
Accession (Uniprot) Number Q03190
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse heart lysate (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and mouse lung lysate (lanes 3 and 6):1-3. Anti-Connexin-37 Antibody (#ACC-204), (1:400).
4-6. Anti-Connexin-37 Antibody, preincubated with Connexin-37 Blocking Peptide (#BLP-CC204). - Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate:1. Anti-Connexin-37 Antibody (#ACC-204), (1:200).
2. Anti-Connexin-37 Antibody, preincubated with Connexin-37 Blocking Peptide (#BLP-CC204).
Last update: 02/01/2024