Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302).
Accession (Uniprot) Number P17302
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes:1. Anti-Connexin-43 Antibody (#ACC-201), (1:400).
2. Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).
Western blot analysis of rat heart membranes:1. Anti-Connexin-43 Antibody (#ACC-201), (1:400).
2. Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).- Rat free floating frozen cerebellum sections (1:300).
Human fetal cochlear sections (Locher, H. et al. (2015) Dev. Neurobiol. 75, 1219.). 
Western blot analysis of rat brain (lanes 1 and 3), (1:1000) and mouse brain (lanes 2 and 4), (1:200) membranes:1,2. Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP).
3,4. Guinea pig Anti-Connexin-43 Antibody, preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).- Mouse brain sections.
Last update: 02/01/2024
