Overview
Type: Synthetic peptide
Form: Lyophilized powder
Connexin-45/GJC1 Blocking Peptide (#BLP-CC207) is the original antigen used for immunization during Anti-Connexin-45 (GJC1) Antibody (#ACC-207) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-45/GJC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1,2. Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:200).
3,4. Anti-Connexin-45 (GJC1) Antibody, preincubated with Connexin-45/GJC1 Blocking Peptide (#BLP-CC207).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:200).
2. Anti-Connexin-45 (GJC1) Antibody, preincubated with Connexin-45/GJC1 Blocking Peptide (#BLP-CC207).
Expression of Connexin-45 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Connexin-45 (GJC1) Antibody (#ACC-207), (1:400), followed by anti-rabbit-Cy3 antibody. Cx45 staining (red) appears in the neuropil of layers stratum oriens and stratum radiatum but not in the pyramidal layer. Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45 (Accession A4GG66).
Accession (Uniprot) Number A4GG66
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
