Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Connexin-47 (GJC2) Blocking Peptide (#BLP-CC222) is the original antigen used for immunization during Anti-Connexin-47 (GJC2) Antibody (#ACC-222) generation. The blocking peptide binds and ‘blocks’ Anti-Connexin-47 (GJC2) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:200).
3-4. Anti-Connexin-47 (GJC2) Antibody, preincubated with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222).
Western blot analysis of human U-87 MG glioblastoma cell line lysate:1. Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:200).
2. Anti-Connexin-47 (GJC2) Antibody, preincubated with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222).
Expression of Connexin-47 in rat spinal cord ventral horn region.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn region showed CX-47 immunoreactivity (green) in neuron profiles (arrows). B. Pre-incubation of the antibody with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Connexin-47 in rat spinal cord dorsal horn region.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CX-47 immunoreactivity (green) appears in the first layers of the dorsal horn (vertical arrows) and in neuron profiles (horizontal arrows). B. Pre-incubation of the antibody with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47 (Accession Q80XF7).
Accession (Uniprot) Number Q80XF7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
