Overview
Type: Synthetic peptide
Form: Lyophilized powder
Contactin-1 (extracellular) Blocking Peptide (#BLP-NR171) is the original antigen used for immunization during Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171) generation. The blocking peptide binds and ‘blocks’ Anti-Contactin-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171), (1:200).
3,4. Anti-Contactin-1/CNTN1 (extracellular) Antibody, preincubated with Contactin-1 (extracellular) Blocking Peptide (#BLP-NR171).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1.Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171), (1:400).
2. Anti-Contactin-1/CNTN1 (extracellular) Antibody, preincubated with Contactin-1 (extracellular) Blocking Peptide (#BLP-NR171).
Expression of Contactin-1 in rat cerebral ventricle:Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Contactin-1 immunoreactivity (green) appears in ependymal cells (arrows) lining the lateral cerebral ventricle (LV). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RPDGQHDGKLFSTHK, corresponding to amino acid residues 947 - 961 of rat Contactin-1 (Accession Q63198).
Accession (Uniprot) Number Q63198
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
