Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Cortactin Blocking Peptide (#BLP-PZ018) is the original antigen used for immunization during Anti-Cortactin Antibody (#APZ-018) generation. The blocking peptide binds and ‘blocks’ Anti-Cortactin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1, 2. Anti-Cortactin Antibody (#APZ-018), (1:1000).
3, 4. Anti-Cortactin Antibody, preincubated with Cortactin Blocking Peptide (#BLP-PZ018).
Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 5), human HeLa cervical adenocarcinoma cell line lysate (lanes 2 and 6), human U-87MG glioblastoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):1-4. Anti-Cortactin Antibody (#APZ-018), (1:200).
5-8. Anti-Cortactin Antibody, preincubated with Cortactin Blocking Peptide (#BLP-PZ018).
Expression of Cortactin in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Cortactin Antibody (#APZ-018), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA3 region, showed immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with Cortactin Blocking Peptide (BLP-PZ018), suppressed staining. Cell nuclei are stained with DAPI (blue). P= pyramidal layer, SR = stratum radiatum.
Properties
Sequence
- Peptide (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin (Accession Q60598).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
