Overview
Type: Synthetic peptide
Form: Lyophilized powder
CPLX1/2 Blocking Peptide (#BLP-NR011) is the original antigen used for immunization during Anti-CPLX1/2 Antibody (#ANR-011) generation. The blocking peptide binds and ‘blocks’ Anti-CPLX1/2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-CPLX1/2 Antibody (#ANR-011), (1:200).
3,4. Anti-CPLX1/2 Antibody, preincubated with CPLX1/2 Blocking Peptide (#BLP-NR011).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-CPLX1/2 Antibody (#ANR-011), (1:200).
2. Anti-CPLX1/2 Antibody, preincubated with CPLX1/2 Blocking Peptide (#BLP-NR011).
Properties
Sequence
- (C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1 (Accession P63041, P84087).
Accession (Uniprot) Number P63041, P84087
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
