Overview
Type: Synthetic peptide
Form: Lyophilized powder
CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050) is the original antigen used for immunization during Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050) generation. The blocking peptide binds and ‘blocks’ Anti-CRF1/CRHR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membrane (lanes 1 and 4), rat brain membrane (lane 2 and 5) and rat cortex lysate (lane 3 and 6):1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).
4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050).
Western blot analysis of human neuroblastoma (SH-SY5Y) (lanes 1 and 4), human brain astrocytoma (CCF-STTGI) (lanes 2 and 5) and rat pheochromocytoma (PC12) (lanes 3 and 6):1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).
4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050).
Expression of Corticotropin-releasing factor receptor 1 in mouse brainImmunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:600). A. CRF1 staining (green) in the cerebellum appears in cerebellar molecular layer (MOL). B. CRF1 staining (green) in the hippocampus appears in soma (horizontal arrows) and dendrites of pyramidal neurons (arrow). In both panels DAPI was used as the counterstain.
Properties
Sequence
- ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1 (Accession P35353).
Accession (Uniprot) Number P35353
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
