Cat #: BLP-CR050
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1 (Accession P35353).
Accession (Uniprot) Number P35353
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse brain membrane (lanes 1 and 4), rat brain membrane (lane 2 and 5) and rat cortex lysate (lane 3 and 6):1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).
4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050). - Mouse brain sections (1:600).
Last update: 02/01/2024