Overview
Type: Synthetic peptide
Form: Lyophilized Powder
CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060) is the original antigen used for immunization during Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060) generation. The blocking peptide binds and ‘blocks’ Anti-CRLR/CALCRL (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 5) and rat heart membranes (lanes 3 and 6):1-3. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200).
4-6. Anti-CRLR/CALCRL (extracellular) Antibody, preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
Western blot analysis of mouse heart lysates (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200).
3, 4. Anti-CRLR/CALCRL (extracellular) Antibody, preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate:1. Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200).
2. Anti-CRLR/CALCRL (extracellular) Antibody, preincubated with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060).
Expression of Calcitonin Receptor-Like Receptor in rat brain stemImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in neurons (arrows). B. Pre-incubation of the antibody with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).
Expression of Calcitonin Receptor-Like Receptor in mouse brain stemImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CRLR/CALCRL (extracellular) (extracellular) Antibody (#ACR-060), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CRLR staining (green) in the Raphe Magnus region is detected in (green) in neurons (arrows). B. Pre-incubation of the antibody with CRLR/CALCRL (extracellular) Blocking Peptide (#BLP-CR060), suppresses staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor (Accession Q16602).
Accession (Uniprot) Number Q16602
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
