Overview
Type: Synthetic peptide
Form: Lyophilized powder
CRMP2 Blocking Peptide (#BLP-IP029) is the original antigen used for immunization during Anti-CRMP2 Antibody (#AIP-029) generation. The blocking peptide binds and ‘blocks’ Anti-CRMP2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI brain astrocytoma (lanes 3 and 7) and rat PC-12 peochromocytoma (lanes 4 and 8) cell line lysates:1-3. Anti-CRMP2 Antibody (#AIP-029), (1:200).
4. Anti-CRMP2 Antibody (1:500).
5-8. Anti-CRMP2 Antibody, preincubated with CRMP2 Blocking Peptide (#BLP-IP029).
Expression of CRMP2 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections sections with Anti-CRMP2 Antibody (#AIP-029), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CRMP2 immunoreactivity (green) appears in the superficial layers of the dorsal horn (arrows). B. Pre-incubation of the antibody with CRMP2 Blocking Peptide (BLP-IP029), suppressed staining. Cell nuclei are stained with DAPI (blue)
Properties
Sequence
- CEVSVTPKTVTPAS, corresponding to amino acid residues 504-517 of rat CRMP2 (Accession P47942).
Accession (Uniprot) Number P47942
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
