Overview
Type: Synthetic peptide
Form: Lyophilized powder
CRTH2/GPR44 (extracellular) Blocking Peptide (#BLP-PR062) is the original antigen used for immunization during Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062) generation. The blocking peptide binds and ‘blocks’ Anti-CRTH2/GPR44 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200).
2. Anti-CRTH2/GPR44 (extracellular) Antibody, preincubated with CRTH2/GPR44 (extracellular) Blocking Peptide (#BLP-PR062).
Western blot analysis of mouse brain lysate:1. Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:400).
2. Anti-CRTH2/GPR44 (extracellular) Antibody, preincubated with CRTH2/GPR44 (extracellular) Blocking Peptide (#BLP-PR062).
Expression of DP2 receptor (CRTH2/GPR44) in rat medial septum and mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. DP2 receptor staining (green) in rat brain sections is detected in neuron-like profiles (arrows) in the medial septum/diagonal band. B. DP2 receptor staining (green) in mouse brain is observed in Purkinje cells (horizontal arrow) and in a subset of neurons in the granule layer (GL, vertical arrow). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2 (Accession Q6XKD3).
Accession (Uniprot) Number Q6XKD3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
