Overview
Type: Synthetic peptide
Form: Lyophilized powder
CX3CL1 (extracellular) Blocking Peptide (#BLP-CR057) is the original antigen used for immunization during Anti-CX3CL1 (extracellular) Antibody (#ACR-057) generation. The blocking peptide binds and ‘blocks’ Anti-CX3CL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membrane (lanes 1 and 5), human pancreatic carcinoma PANC-1 lysate (lanes 2 and 6), mouse heart membrane (lanes 3 and 7) and rat brain synaptosomal fraction (lanes 4 and 8):1-4. Anti-CX3CL1 (extracellular) Antibody (#ACR-057), (1:400).
5-8. Anti-CX3CL1 (extracellular) Antibody, preincubated with CX3CL1 (extracellular) Blocking Peptide (#BLP-CR057).
Expression of CX3CL1 in rat frontal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CX3CL1 (extracellular) Antibody (#ACR-057), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CX3CL1 staining (green) in the deep layer of the frontal cortex is detected in cortical neurons (vertical arrows) and their dendrites (horizontal arrows). B. CX3CL1 immunoreactivity (green) in the upper layer of the frontal cortex is observed in cortical neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CGKRAIILETRQHR, corresponding to amino acid residues 58-71 of human CX3CL1 (Accession P78423).
Accession (Uniprot) Number P78423
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
