Overview
Cat #: BLP-CR014
Type: Synthetic peptide
Form: Lyophilized powder
CXCR4 (extracellular) Blocking Peptide (#BLP-CR014) is the original antigen used for immunization during Anti-CXCR4 (extracellular) Antibody (#ACR-014) generation. The blocking peptide binds and ‘blocks’ Anti-CXCR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:200).
3,4. Anti-CXCR4 (extracellular) Antibody, preincubated with CXCR4 (extracellular) Blocking Peptide (#BLP-CR014). - Expression of CXCR4 in rat cerebellumImmunohistochemical staining of rat cerebellum frozen section using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:100). CXCR4 is expressed in Purkinje cell bodies and axonal prolongations (arrows). Hoechst 33342 is used as the counterstain.
- Expression of CXCR4 in rat brainImmunohistochemical staining of rat temporal cortex using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:400) followed by donkey anti-rabbit-biotin and streptavidin labeled with Cy3. A. CXCR4 staining (red) appears in neuronal soma and processes (arrows). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)EGISIYTSDNYTEE, corresponding to amino acid residues 2-15 of human CXCR4 (Accession P61073).
Accession (Uniprot) Number P61073
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.