Overview
Type: Synthetic peptide
Form: Lyophilized powder
CysLTR1 (extracellular) Blocking Peptide (#BLP-LR003) is the original antigen used for immunization during Anti-CysLTR1 (extracellular) Antibody (#ALR-003) generation. The blocking peptide binds and ‘blocks’ Anti-CysLTR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute T-cell leukemia Jurkat (lanes 3 and 6) human cell lysates:1,2,3. Anti-CysLTR1 (extracellular) Antibody (#ALR-003), (1:200).
4,5,6. Anti-CysLTR1 (extracellular) Antibody, preincubated with CysLTR1 (extracellular) Blocking Peptide (#BLP-LR003).
Properties
Sequence
- (C)HNETKP(S)DSVLRMQK, corresponding to amino acid residues 261-275 of human CysLTR1 (Accession Q9Y271).
Accession (Uniprot) Number Q9Y271
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
