Cat #: BLP-DR001
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901).
Accession (Uniprot) Number P18901
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-D1 Dopamine Receptor Antibody (#ADR-001), (1:200).
3,4. Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001). - Rat brain sections (frozen), (1:100).
- Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):1,2. Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP), (1:400).
3,4. Guinea pig Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001). - Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
1. Guinea pig Anti-D1 Dopamine Receptor Antibody (#ADR-001-GP), (1:400).
2. Guinea pig Anti-D1 Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001). - Rat brain sections (1:300).
Last update: 02/01/2024