Overview
Type: Synthetic peptide
Form: Lyophilized powder
D2 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR002) is the original antigen used for immunization during Anti-D2 Dopamine Receptor (extracellular) Antibody (#ADR-002) generation. The blocking peptide binds and ‘blocks’ Anti-D2 Dopamine Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat striatum (lanes 1 and 5) and hippocampus (lanes 2 and 6) membranes and of rat (lanes 3 and 7) and mouse (lanes 4 and 8) whole brain lysates:1-4. Anti-D2 Dopamine Receptor (extracellular) Antibody (#ADR-002), (1:200).
5-8. Anti-D2 Dopamine Receptor (extracellular) Antibody, preincubated with D2 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR002).Rat brain sections (1:100), (see Yang, Y.L. et al. (2019) Front. Neurosci. 13, 195.).
Properties
Sequence
- (C)DDLERQNWSRPFNGSE, corresponding to amino acid residues 11-26 of rat DRD2 (Accession P61169).
Accession (Uniprot) Number P61169
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
