Overview
Type: Synthetic peptide
Form: Lyophilized powder
D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003) is the original antigen used for immunization during Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003) generation. The blocking peptide binds and ‘blocks’ Anti-D3 Dopamine Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of whole rat brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes:1,2. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).
3,4. Anti-D3 Dopamine Receptor (extracellular) Antibody, preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).
Western blot analysis of mouse brain membranes:1. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).
2. Anti-D3 Dopamine Receptor (extracellular) Antibody, preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).
Expression of DRD3 in rat striatumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:100). A. DRD3 appears in a subset of striatal neurons (green). B. The same section was stained for parvalbumin (red), a marker of interneurons. C. Merging the two images demonstrates that DRD3 localization includes parvalbuminergic striatal interneurons. DAPI was used as the counterstain (blue).
Properties
Sequence
- CGAENSTGVNRARPH, corresponding to amino acid residues 15-29 of rat DRD3 (Accession P19020).
Accession (Uniprot) Number P19020
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
