Overview
Type: Synthetic peptide
Form: Lyophilized powder
D4 Dopamine Receptor Blocking Peptide (#BLP-DR004) is the original antigen used for immunization during Anti-D4 Dopamine Receptor Antibody (#ADR-004) generation. The blocking peptide binds and ‘blocks’ Anti-D4 Dopamine Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-D4 Dopamine Receptor Antibody (#ADR-004), (1:200).
3,4. Anti-D4 Dopamine Receptor Antibody, preincubated with D4 Dopamine Receptor Blocking Peptide (#BLP-DR004).
Properties
Sequence
- (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4 (Accession P30729).
Accession (Uniprot) Number P30729
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
