Overview
Type: Synthetic peptide
Form: Lyophilized powder
D5 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR005) is the original antigen used for immunization during Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005) generation. The blocking peptide binds and ‘blocks’ Anti-D5 Dopamine Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes:1. Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005), (1:200).
2. Anti-D5 Dopamine Receptor (extracellular) Antibody, preincubated with D5 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR005).
Western blot analysis of rat striatum membranes:1. Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005), (1:200).
2. Anti-D5 Dopamine Receptor (extracellular) Antibody, preincubated with D5 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR005).
Expression of D5 dopamine receptor in rat striatumImmunohistochemical staining of perfusion-fixed rat brain frozen using Anti-D5 Dopamine Receptor (extracellular) Antibody (#ADR-005), (1:100). A. DRD5 (green) appears in a subset of striatal neurons and in the striatal matrix. B. Staining of the same section with calbindin D28k (red), a marker of interneurons. C. Merging the two images demonstrates that DRD5 partially overlaps with the population of calbindin containing striatal interneurons. DAPI is used as the counterstain (blue).
Properties
Sequence
- EEGWELEGRTENC, corresponding to amino acid residues 199-211 of rat DRD5 (Accession P25115).
Accession (Uniprot) Number P25115
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
