Overview
Type: Synthetic peptide
Form: Lyophilized powder
DLGAP1/GKAP Blocking Peptide (#BLP-PZ041) is the original antigen used for immunization during Anti-DLGAP1/GKAP Antibody (#APZ-041) generation. The blocking peptide binds and ‘blocks’ Anti-DLGAP1/GKAP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200).
3,4. Anti-DLGAP1/GKAP Antibody, preincubated with DLGAP1/GKAP Blocking Peptide (#BLP-PZ041).
Expression of GKAP in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GKAP staining (green) in hippocampal CA3 region is detected in neurons (arrows) of CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KISNEHSPKLQIRSH, corresponding to amino acid residues 397-411 of rat DLGAP1 (Accession P97836).
Accession (Uniprot) Number P97836
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
