Overview
Alternative Name Taxotere, RP 56976
Lyophilized Powder yes
Origin Taxus brevifolia (Pacific Yew tree).
Source Semi-synthetic
MW: 807.89
Purity: >98%.
Effective concentration 1-100 nM.
Structure
Chemical name N-Debenzoyl-N-tert-butoxycarbonyl-10-deacetyl taxol.
Molecular formula C43H53NO14.
CAS No.: 114977-28-5
Activity Docetaxel, derived from Paclitaxel, inhibits the function of tubulin by binding to β-tubulin. Since it stabilizes microtubules, it is anti-mitotic, induces apoptosis and acts as a neoplastic agent1-3.
References-Activity
- Parness, J. and Horwitz, S.B. (1981) J. Cell Biol. 91, 479.
- Ringel, I. and Horwitz, S.B. (1989) J. Natl. Cancer Inst. 83, 288.
- Gueritte-Voegelein, F. et al. (1991) J. Med. Chem. 34, 992.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or six months at -20°C.
Our bioassay
Alomone Labs Docetaxel prevents cell division and induces the formation of microtubules bundles HUVEC cytoplasm.Cells were treated with vehicle (A) or with 10nM (B) and 100 nM (C) Docetaxel (#D-800) for 22 h. The cells were then fixed with 4% PFA and probed with anti-β-tubulin followed by a Rhodamine-conjugated secondary antibody (2). DNA was stained with Hoechst 33342 (1). Image (3) of each treatment is the digital overlay of images (1) and (2). B. A multi-nuclear cell is caused by low concentrations of Docetaxel (arrows, panel 1) and microtubule bundles are caused by higher concentrations of Docetaxel (C, arrows, panel 2).
Lyophilized Powder
For research purposes only, not for human use
Last Update: 30/03/2021
Specifications
Citations
Citations
