Overview
Type: Synthetic peptide
Form: Lyophilized powder
Dopamine Transporter/DAT (extracellular) Blocking Peptide (#BLP-MT003) is the original antigen used for immunization during Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003) generation. The blocking peptide binds and ‘blocks’ Anti-Dopamine Transporter/DAT (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 4) and mouse (lanes 2 and 5) brain membranes and rat pheochromocytoma PC12 (lanes 3 and 6) cell lysates:1-3. Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003), (1:200).
4-6. Anti-Dopamine Transporter (DAT) (extracellular) Antibody, preincubated with Dopamine Transporter/DAT (extracellular) Blocking Peptide (#BLP-MT003).- Mouse coronal brain sections (1:200) (Hong, J. et al. (2015) Cell Death Dis. 6, e1832.).
Properties
Sequence
- (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT (Accession P23977).
Accession (Uniprot) Number P23977
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
