Overview
Type: Synthetic peptide
Form: Lyophilized powder
DPP6 (extracellular) Blocking Peptide (#BLP-PC146) is the original antigen used for immunization during Anti-DPP6 (extracellular) Antibody (#APC-146) generation. The blocking peptide binds and ‘blocks’ Anti-DPP6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:1,2. Anti-DPP6 (extracellular) Antibody (#APC-146), (1:200).
3,4. Anti-DPP6 (extracellular) Antibody, preincubated with DPP6 (extracellular) Blocking Peptide (#BLP-PC146).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Anti-DPP6 (extracellular) Antibody (#APC-146), (1:500).
2. Anti-DPP6 (extracellular) Antibody, preincubated with DPP6 (extracellular) Blocking Peptide (#BLP-PC146).
Multiplex staining of DPP6 and KV4.2 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-DPP6 (extracellular) Antibody (#APC-146), (1:400) and Guinea pig Anti-KV4.2 Antibody (#APC-023-GP), (1:1000). A. DPP6 (green) is expressed in the CA3 rat hippocampal region. B. KV4.2 (red) is expressed in the same region. C. Merge of the two images demonstrates partial colocalization in neurons of the CA3 region (arrows). Cell nuclei are stained with DAPI (blue).
Expression of DPP6 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-DPP6 (extracellular) Antibody (#APC-146), (1:400), followed by donkey anti-rabbit-Cy3 antibody (red). Staining in the hippocampal CA3 region appears in interneurons (arrows). DAPI is used to stain nuclei (blue).
Properties
Sequence
- (C)KKHEDESEAWLHR, corresponding to amino acid residues 414-426 of human DPP6 (Accession P42658).
Accession (Uniprot) Number P42658
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
