Overview
Type: Synthetic peptide
Form: Lyophilized Powder
DSCAM (extracellular) Blocking Peptide (#BLP-NR046) is the original antigen used for immunization during Anti-DSCAM (extracellular) Antibody (#ANR-046) generation. The blocking peptide binds and ‘blocks’ Anti-DSCAM (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain synaptosomes lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-DSCAM (extracellular) Antibody (#ANR-046), (1:200).
3-4. Anti-DSCAM (extracellular) Antibody, preincubated with DSCAM (extracellular) Blocking Peptide (BLP-NR046).
Western blot analysis of human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):1-2. Anti-DSCAM (extracellular) Antibody (#ANR-046), (1:200).
3-4. Anti-DSCAM (extracellular) Antibody, preincubated with DSCAM (extracellular) Blocking Peptide (BLP-NR046).
Expression of DSCAM in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DSCAM (extracellular) Antibody (#ANR-046), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. DSCAM immunoreactivity (green), appears in cells of the purkinje layer (arrows) and distributed diffusely in the molecular layer (M). B. Pre-incubation of the antibody with DSCAM (extracellular) Blocking Peptide (BLP-NR046), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer, M = molecular layer.
Properties
Sequence
- Peptide (C)RITGLNHANLIMDHMVK, corresponding to amino acid residues 361 - 377 of mouse DSCAM (Accession Q9ERC8).
Accession (Uniprot) Number Q9ERC8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
