Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KQFKTSYEKRSFK, corresponding to amino acids 188-200 of rat EAAT1 (Accession P24942).
Accession (Uniprot) Number P24942
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021) (1:500).
3,4. Anti-EAAT1 (GLAST) (extracellular) Antibody, preincubated with EAAT1/GLAST (extracellular) Blocking Peptide (#BLP-GC021).- Rat brain sections (frozen) (1:200).
Last update: 02/01/2024
