Overview
Type: Synthetic peptide
Form: Lyophilized powder
EAAT2/GLT-1 (extracellular) Blocking Peptide (#BLP-GC022) is the original antigen used for immunization during Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022) generation. The blocking peptide binds and ‘blocks’ Anti-EAAT2/GLT-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cerebellum lysate:1. Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:500).
2. Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2/GLT-1 (extracellular) Blocking Peptide (#BLP-GC022).
Western blot analysis of mouse brain membranes:1. Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:200).
2. Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2/GLT-1 (extracellular) Blocking Peptide (#BLP-GC022).
Expression of EAAT2 in rat hippocampusImmunohistochemical staining of perfusion-fixed, free floating frozen rat brain sections with Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022). In the rat hippocampal CA1 region, EAAT2 immunoreactivity (green) appears in the neuropil in the stratum oriens (S. Or) and in the pyramidal layer (Pyr) and was particularly intense around pyramidal neurons (arrow in A and compare with DAPI counterstain in B).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022-GP), (1:800).
3-4. Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2 (GLT-1) (extracellular) Blocking Peptide (BLP-GC022).
Expression of EAAT2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022-GP), (1:100), followed by goat anti-guinea pig-AlexaFluor-594. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (red) in the hilus (H) subgranular layer (vertical arrows) and between cells in the granule layer (G) (horizontal arrows). B. Pre-incubation of the antibody with EAAT2 (GLT-1) (extracellular) Blocking Peptide (BLP-GC022), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of EAAT2 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022-GP), (1:100), followed by goat anti-guinea pig-AlexaFluor-594. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (red) in the hilus (H) subgranular layer (vertical arrows) and between cells in the granule layer (G) (horizontal arrows). B. Pre-incubation of the antibody with EAAT2 (GLT-1) (extracellular) Blocking Peptide (BLP-GC022), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KQLGPGKKNDEVS, corresponding to amino acid residues 151-163 of rat EAAT2 (Accession P31596).
Accession (Uniprot) Number P31596
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
