Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KQLGPGKKNDEVS, corresponding to amino acid residues 151-163 of rat EAAT2 (Accession P31596).
Accession (Uniprot) Number P31596
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cerebellum lysate:1. Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:500).
2. Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2/GLT-1 (extracellular) Blocking Peptide (#BLP-GC022).
Western blot analysis of mouse brain membranes:1. Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:200).
2. Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2/GLT-1 (extracellular) Blocking Peptide (#BLP-GC022).- Rat brain sections (frozen) (1:200).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022-GP), (1:800).
3-4. Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody, preincubated with EAAT2 (GLT-1) (extracellular) Blocking Peptide (BLP-GC022).
Last update: 02/01/2024
