Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)EPTILDNEDSDTKK, corresponding to amino acid residues 487-500 of rat EAAT3 (Accession P51907).
Accession (Uniprot) Number P51907
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-EAAT3 (EAAC1) Antibody (#AGC-023), (1:400).
2. Anti-EAAT3 (EAAC1) Antibody, preincubated with EAAT3/EAAC1 Blocking Peptide (#BLP-GC023).
Western blot analysis of mouse kidney lysate:1. Anti-EAAT3 (EAAC1) Antibody (#AGC-023), (1:400).
2. Anti-EAAT3 (EAAC1) Antibody, preincubated with EAAT3/EAAC1 Blocking Peptide (#BLP-GC023).- Rat brain sections (frozen), (1:200-1:400).
Last update: 02/01/2024
