Overview
Type: Synthetic peptide
Form: Lyophilized powder
EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051) is the original antigen used for immunization during Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) generation. The blocking peptide binds and ‘blocks’ Anti-EMR1/ADGRE1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat fat (lanes 1 and 6), mouse fat (lanes 2 and 7), human Burkitt's lymphoma (Raji) (lanes 3 and 8), rat basophilic leukemia (RBL) (lanes 4 and 9) and human promyelocytic leukemia (HL-60) (lanes 5 and 10) lysates:1-5. Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), (1:400).
6-10. Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Western blot analysis of human promyelocytic leukemia (HL-60) (lanes 1 and 5), human Burkitt's lymphoma (Raji) B (lanes 2 and 6), mouse macrophage J774 (lanes 3 and 7) and rat basophilic leukemia (RBL) (lanes 4 and 8) cell lysates:1-4. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
5-8. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Western blot analysis of rat (lanes 9 and 11) and mouse (lanes 10 and 12) fat tissue lysate:9, 10. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
11, 12. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Western blot analysis of rat (lanes 13
and 15) and mouse (lanes 14 and 16) spleen lysate:13, 14. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
15, 16. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Properties
Sequence
- CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246).
Accession (Uniprot) Number Q14246
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
