Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246).
Accession (Uniprot) Number Q14246
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat fat (lanes 1 and 6), mouse fat (lanes 2 and 7), human Burkitt's lymphoma (Raji) (lanes 3 and 8), rat basophilic leukemia (RBL) (lanes 4 and 9) and human promyelocytic leukemia (HL-60) (lanes 5 and 10) lysates:1-5. Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), (1:400).
6-10. Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Western blot analysis of human promyelocytic leukemia (HL-60) (lanes 1 and 5), human Burkitt's lymphoma (Raji) B (lanes 2 and 6), mouse macrophage J774 (lanes 3 and 7) and rat basophilic leukemia (RBL) (lanes 4 and 8) cell lysates:1-4. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
5-8. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Last update: 02/01/2024
