Overview
Type: Synthetic peptide
Form: Lyophilized powder
ENaC β/SCNN1B (extracellular) Blocking Peptide (#BLP-SC019) is the original antigen used for immunization during Anti-ENaC β (SCNN1B) (extracellular) Antibody (#ASC-019) generation. The blocking peptide binds and ‘blocks’ Anti-ENaC β/SCNN1B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and lung (lanes 2 and 4) lysates:1,2. Anti-ENaC β (SCNN1B) (extracellular) Antibody (#ASC-019), (1:200).
3,4. Anti-ENaC β (SCNN1B) (extracellular) Antibody, preincubated with ENaC β/SCNN1B (extracellular) Blocking Peptide (#BLP-SC019).- Mouse kidney (1:200) (Yang, S.S. et al. (2010) Endocrinology 151, 1829.).
Properties
Sequence
- EFNYRTIEESPANNI(C), corresponding to amino acid residues 498-513 of rat ENaCβ (Accession P37090).
Accession (Uniprot) Number P37090
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
