Overview
Type: Synthetic peptide
Form: Lyophilized powder
ENaC gamma/SCNN1G (extracellular) Blocking Peptide (#BLP-SC011) is the original antigen used for immunization during Anti-ENaC γ (SCNN1G) (extracellular) Antibody (#ASC-011) generation. The blocking peptide binds and ‘blocks’ Anti-ENaC gamma/SCNN1G (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung lysate:1. Anti-ENaC γ (SCNN1G) (extracellular) Antibody (#ASC-011), (1:200).
2. Anti-ENaC γ (SCNN1G) (extracellular) Antibody, preincubated with ENaC gamma/SCNN1G (extracellular) Blocking Peptide (#BLP-SC011).- Guinea pig inner ear sections (1:300) (Mori, Y. et al. (2009) J. Physiol. Sci. 59, 355.).
Properties
Sequence
- (C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaCγ (Accession P37091).
Accession (Uniprot) Number P37091
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
