Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Endothelin Receptor A (extracellular) Blocking Peptide (#BLP-ER004) is the original antigen used for immunization during Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004) generation. The blocking peptide binds and ‘blocks’ Anti-Endothelin Receptor A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004), (1:200).
3-4. Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004), preincubated with Endothelin Receptor A (extracellular) Blocking Peptide (BLP-ER004).
Western blot analysis of mouse lung lysate:1. Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004), (1:200).
2. Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004), preincubated with Endothelin Receptor A (extracellular) Blocking Peptide (BLP-ER004).
Western blot analysis of human HUVEC endothelial cell line lysate (lanes 1 and 4), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 5) and human U-87 MG glioblastoma cell line lysate (lanes 3 and 6):1-3. Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004), (1:400).
4-6. Anti-Endothelin Receptor A (extracellular) Antibody (#AER-004), preincubated with Endothelin Receptor A (extracellular) Blocking Peptide (BLP-ER004).
Properties
Sequence
- Peptide KKTVYDEMDKNRC, corresponding to amino acid residues 329 - 341 of rat EDNRA (Accession P26684).
Accession (Uniprot) Number P26684
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
