Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)NHNTERSSHKDSMN, corresponding to amino acid residues 413-426 of rat ET-A (Accession P26684).
Accession (Uniprot) Number P26684
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Endothelin Receptor A Antibody (#AER-001), (1:200).
2. Anti-Endothelin Receptor A Antibody, preincubated with Endothelin Receptor A Blocking Peptide (#BLP-ER001).- Rat brain sections and rat lung paraffin embedded section (1:100). Mouse retinas (Bramall, A.N. et al. (2013) PLoS ONE 8, e58023.)
Last update: 02/01/2024
